Department of Integrative and Systems Physiology
Our laboratory seek to clarify mechanisms of life at molecular, organelle, cellular, organic and body levels with a new approach 'Systems Physiology' combining physiological experiments 'wet experiments' and mathematical analysis 'dry experiments'.
I am interested in how functions of cell, organ and body are organized
by orchestrating various elements (proteins) especially in heart. We challenge
this problem by performing a multi-level experiments combined with mathematical
analysis. Molecular biological study, electrophysiological study, fluorescence
imaging, motion analysis as well as mathematical modeling and analysis
are used. With this approach, we clarified how individual ion channels/transporters
play in cardiac excitation-contraction coupling, rhythm generation of heart
(automaticity) and cell volume regulation. The representative mathematical
model is a model of comprehensive cardiac myocyte, Kyoto model.
We investigate also functions of non-excitable cells, such as lymphocytes. We study how ion channels/transporters, especially mitochondria Ca transporters, regulate lymphocyte response to antigen.
We welcome all who are interested in systems physiology and want to challenge the logic of life.
I am studying cellular ion dynamics of cardiomyocytes and lymphocytes by a combination of experiments and simulation analysis.
I am interested in single-molecule dynamics of ion channel proteins. Conformational changes of the proteins were recorded by diffracted x-ray tracking, which can track the motions as a movie. I am trying to reveal the dynamics of the ion channel proteins related to their function by combining the developments of the method and the data analysis.
I have been interested in local Ca signalings in wide variety of cell types
(e.g. pacemaker cells within GI tract, vascular smooth muscle cells, and
pancreatic beta cells) and their relationships to distinct cellular functions,
especially when Ca dynamics are modulated by second messengers. In this
lab, I am focusing on cardiac pacemaker cells and lymphocytes. By combining
experiments with biosimulation analysis, I am challenging to reveal how
local Ca handlings between mitochondria and ER at submembrane spaces control
automaticity of the pacemaker cells and lymphocyte response to antigen,
particularly when different types of intracellular signaling cascades are
I am enthusiastic about how life is mastered by complex activities of biomolecules and this enchantment influenced me to develop my career in the direction of academic teaching and research on medical science. As a PhD research student I am now working on the molecular mechanisms and roles of mitochondrial Ca2+ transporters especially the Na+-Ca2+ exchange system (mediated via NCLX) under the guidance of Professor Satoshi Matsuoka.
I am a secretary of this laboratry.
Title: Analysis of relationship between migration and calcium dynamics
in a tumor cell by using a cell migration chemotactic control technology
(supervised by Prof. Matsuoka and Prof. Fujita).
It has been reported that a gradient of intracellular calcium concentration is produced during migration or chemotaxis of a kind of cells. However, it is not clarified whether the same phenomenon occurs in tumor cells. Therefore, I want to clarify the relationship between cell migration and calcium dynamics in tumor cells by using a cell migration chemotactic control technology. In addition to this, I do hope to discover unknown mechanisms underlying tumor cell migration.
Members of Fukui Medical High School have studied the contribution of mitochondrial Ca2+ transporter to action potential generation of mice ventricular myocytes.: Yousuke, Saki, Erika and Rena
We study the Ca2+ dynamics of mouse ventricular myocyte: Misaki, Moe and Shino.
We investigated the effects of empagliflozin, an antidiabetic agent, on mouse ventricular myocytes : Shunsuke, Hinako, Takuma, Saki.
Using isolated cardiomyocytes, we are investigating the role of extracellular ion concentration in Ca2 + transient: Marin, Masaki, Ryoya, Takeru.
Akiko Takaku (2014-2017)
Naoko Takaku (2015-2016)